We are interested in understanding the mechanisms underlying remodelling of the actin cytoskeleton. Force generation by actin polymerisation is important for fundamental cell biological activities such as cell motility, cell shape change, vesicle trafficking, cytokinesis. Alterations in actin-based cell protrusions and movement are a characteristic difference in metastasizing cancer cells compared with normal cells. We use in vitro reconstitution systems, advanced microscopy and model organisms to understand how signalling, proteins and lipids that regulate the actin cytoskeleton orchestrate actin dynamics.