The Regulation of Replication Initiation in Eukaryotes Faithful inheritance of the genome from mother to daughter cell requires that it is replicated accurately, in its entirety, exactly once. Failure to perfectly replicate the genome is a likely source of cell death and the mutations that are the root cause of cancer. As a result, replication is strictly regulated both within the cell cycle and in the face of DNA lesions and it is these levels of regulation that form the focus of our research interests.