28 November 2018
Genome integrity is continuously threatened by DNA damage arising from both exogenous and endogenous sources. In response to DNA damage cells activate a signal transduction cascade, known as the DNA damage checkpoint, that results in the activation of DNA repair pathways. Timely termination of this signalling cascade is then essential for the resumption of normal cell growth once DNA damage has been repaired.
A new study from Professor Tony Kouzarides’ group in collaboration with Professor Steve Jackson’s group, both at the Gurdon Institute, shows how this transition between DNA damage checkpoint activation and termination is regulated in baker’s yeast. The authors identified a DNA-damage-regulated histone modification in Saccharomyces cerevisiae, phosphorylation of histone H4 threonine 80, and showed that it triggers DNA damage checkpoint termination by promoting the timely recruitment of Rtt107 protein to displace the checkpoint adaptor Rad9, thereby interrupting the checkpoint-signalling cascade.
It is well known that the DNA damage checkpoint acts as a barrier during early tumourigenesis, and that its inactivation enhances tumour progression. Therefore, understanding the mechanisms by which cells inactivate the checkpoint-signalling cascade may reveal new potential therapeutic targets for cancer treatment.
31 October 2018
The causes of psychiatric diseases can be multiple, but certainly involve both environmental and genetic aspects. A new study from Professor Eric Miska’s Group in our Programme, in collaboration with Professor Isabelle Mansuy at ETH/University of Zürich, presents compelling evidence for a new player in the mechanisms that mediate environmentally increased disease risk. Using a well-established mouse model, the authors demonstrate that early life trauma alters long RNA molecules in sperm. These molecules relay the effects of the traumatic experience on anxiety, insulin metabolism and food intake to the offspring complementary to altered small RNAs, that convey effects on depressive-like behavior.
These findings have strong implications for potential intervention strategies targeting the prevention of the inheritance of trauma-induced increased disease risk.
29 October 2018
Both the formation and function of the epithelial sheets that form most of our organs depends on the apical-basal (top-bottom) polarisation of each cell, whereas a loss of this polarity is a hallmark for cancer. It has generally been assumed that all epithelia are polarised by a common mechanism involving a conserved set of polarity factors. In their new publication, Professor Daniel St Johnston’s Group at the Gurdon Institute show that none of these factors are required for polarity in the fly midgut epithelium, indicating that there are at least two types of epithelia that polarise by fundamentally different mechanisms. Furthermore, their findings reveal that the Drosophila midgut is more similar to most mammalian epithelia than other fly epithelia, suggesting that it will be a good model for studying how cells within epithelial sheets polarise and how this polarity is disturbed in cancer.
24 October 2018
Scientific literature on cancer is huge, and with thousands of new publications appearing each month, it has become increasingly difficult for cancer researchers to stay up to date with developments in their own field. Researchers from Professor Anna Korhonen’s Group at the University of Cambridge Language Technology Laboratory have teamed up with the groups of Dr Masashi Narita at the CRUK Cambridge Institute and Professor Ulla Stenius at the Karolinska Institutet in Sweden to create a new computational tool, called LION LBD, that addresses this problem. The tool, based on AI technology, not only helps scientists keep abreast with published discoveries on cancer, but also helps them make new discoveries by combining what is already known in published literature. Facilitating large-scale hypothesis testing and generation from huge collections of biomedical literature, LION LBD is the first literature-based discovery system developed for the needs of cancer biology. It is freely available at http://lbd.lionproject.net.
14 September 2018
RNA viruses are often considered to present the highest threat for triggering a worldwide pandemic. In their new Nature Methods paper, researchers from Professor Eric Miska’s group and international collaborators describe a new technique to determine the structure of the Zika virus RNA genome while replicating inside human cells, as well as to explore how Zika virus interacts with the host's own RNA. The technique is called COMRADES (Crosslink Of Matched RNAs And DEep Sequencing) and, importantly, it can be applied to any RNA virus in any host cell and provides insight into how viruses direct the host cell to create new virus particles. COMRADES could also allow researchers to design artificial molecules capable of blocking the virus replication process and preventing the virus spreading.
12 September 2018
RNA viruses are a major threat to animals and plants. RNA interference (RNAi) and the interferon response provide innate antiviral defense against RNA viruses. In their new publication, Professor Eric Miska's Group and their collaborators performed a large-scale screen, using Caenorhabditis elegans and its natural pathogen the Orsay virus (OrV), which identified cde-1 as important for antiviral defense. CDE-1 is a homolog of the mammalian TUT4 and TUT7 terminal uridylyltransferases (collectively called TUT4(7)); its catalytic activity is required for its antiviral function. CDE-1 uridylates the 3' end of the OrV RNA genome and promotes its degradation in a manner independent of the RNAi pathway. Likewise, TUT4(7) enzymes uridylate influenza A virus (IAV) mRNAs in mammalian cells. Deletion of TUT4(7) leads to increased IAV mRNA and protein levels. Collectively, these data implicate 3'-terminal uridylation of viral RNAs as a conserved antiviral defense mechanism.
17 August 2018
CRISPR-Cas9 is a powerful technology with important applications in cancer research, as it can help identify genes that are essential for cancers to grow. However, cancer cells can accumulate extra copies of specific genes, which are then wrongly classed as being essential for cancer. Researchers from the Wellcome Sanger Institute have created a new computational tool, called CRISPRcleanR, that accounts for this undesired bias in the data derived from CRISPR-Cas9 screens.
Published in BMC Genomics and as part of the Cancer DepMap at the Wellcome Sanger Institute, the method allows better interpretation and prioritising of gene hits for the development of new anti-cancer therapies. Notably, this approach does not require any information about the number of extra gene copies. The method will improve the analysis of CRISPR-Cas9 knockout screens to identify essential cancer genes.
7 August 2018
Researchers at the MRC Cancer Unit have shown how a set of proteins can change cancer cell behaviour. Using computer simulations, they discovered how coordination between a network of membrane transporters can be exploited to improve existing drug action, and potentially generate new treatments. These transporters are used to maintain the balance of chemicals inside and outside the cell. The gradients of these chemicals are then used in processes like cell size maintenance, and control fundamental behaviours such as cell movement and growth.
In their new publication, researchers from Dr Ben Hall’s group show for the first time that alterations in the expression of these membrane transporters consistently occurs in all cancers. Going further, the researchers were able to construct a computational model of the key chemical gradients and transporters within a cell, and show precisely how changes in the expression of them can alter cancer cell behaviour. This research is a start at understanding how these proteins can be used as potential markers or drug targets in the future.
23 July 2018
Autophagy is a process of cellular self-digestion that is required to prevent the build-up of damaged molecules that would otherwise predispose to a range of diseases including neurodegeneration and cancer. In their new publication, researchers from Dr Masashi Narita’s Group at the CRUK Cambridge Institute, and their collaborators, describe a new model in which we can control when and where autophagy happens. Instead of completely deleting a gene, they developed a system that allows us to turn off and on a key autophagy gene, blocking and re-establishing autophagy, respectively. This has enabled them to perform experiments that were previously impossible and uncover novel insights. In one example, the researchers were able to show that autophagy inhibition can drive liver damage, yet liver fibrosis occurred only after autophagy restoration. Such models are necessary to test the role of autophagy at different stages of disease, such as in cancer where it may have different roles in tumour initiation, progression, and treatments.
20 July 2018
Breast cancer is the most common cancer in the UK, with up to 10% of hereditary cases due to inheriting a faulty cancer-causing gene, for example, mutations in the BRCA1 gene. Individuals with BRCA1 mutations are at high risk of developing breast cancer, and often opt for prophylactic breast removal, as was reported for Hollywood actress Angelina Jolie. Previous research in Professor Steve Jackson’s Group at the Gurdon Institute led to the development of PARP inhibitors, a new class of cancer therapy which is highly effective in treating cancers with these mutations. Unfortunately, drug resistance is a common response, and so his group set out to establish how this resistance might develop.
Using state-of-the-art CRISPR gene editing technology, the researchers scanned the human genome for factors which, when mutated, could cause drug resistance in cells that lacked BRCA1. One of these factors was the previously uncharacterised Shieldin complex.
BRCA1 is critical for performing the accurate type of DNA repair known as homologous recombination (HR). This is counterbalanced by an opposing ‘error-prone’ repair pathway known as non-homologous end-joining (NHEJ). The researchers identified Shieldin as a new component of the NHEJ pathway. BRCA1-negative cells rely on this error-prone DNA repair pathway, which makes them susceptible to PARP inhibitors. If Shieldin is removed from these cells, the imbalance between the repair pathways is reversed, restoring the ability of the cell to perform DNA repair by HR, overcoming the toxicity of PARP inhibitors and therefore leading to drug resistance. The study went on to show that resistance to PARP inhibitors can lead the same cancer cells to develop vulnerabilities to alternative cancer treatments, such as radiotherapy or platinum-based chemotherapy.
11 July 2018
One crucial part of human cells is a structure called the nuclear envelope, which surrounds the cell nucleus – home to our DNA. This structure is essential for maintaining normal nuclear architecture and cell function. Dysfunction of the nuclear envelope leads to various human diseases, including the rare, but devastating Hutchinson-Gilford progeria syndrome (HGPS) for which there is no current cure. In their new publication, Professor Steve Jackson’s Group at the Wellcome Trust Gurdon Institute and Dr Delphine Larrieu’s Group at the Cambridge Institute for Medical Research identified a new pathway – the Transportin-1 pathway responsible for importing several proteins into the nucleus – as being affected in HGPS. These proteins are involved in important cellular processes including organisation of DNA structure and regulation of gene expression. Consequently, in HGPS, they cannot effectively reach the nucleus and are therefore unable to play their normal roles. The authors show that by targeting a protein called NAT10, we can restore the Transportin-1 pathway in HGPS cells, leading to improvement of DNA structure, gene expression re-balancing, and delayed entry of cells into senescence.
9 July 2018
Transmissible cancers are clonal cell lineages that can spread between individuals via transfer of living cancer cells. One of the oldest known contagious cancers is Canine Transmissible Venereal Tumour (CTVT). CTVT is usually sexually-transmitted between dogs and manifests as genital tumours. This dogged disease first arose from the cells of an early domesticated dog thousands of years ago and has since then "metastasized" through global dog populations. In their new study, Dr Elizabeth Murchison’s Group at the Department of Veterinary Medicine trace the historic origin of this cancer lineage using samples of DNA from ancient canids. The study reveals that CTVT first arose over eight thousand years ago in a “founder” individual very closely related to an extinct population of dogs that were once widespread across the Americas.
4 July 2018
Our body consists of various organs, which are further composed of tissues that each include diverse populations of highly specialised cells. As such, it is important to precisely look at how each cell regulates its genes in order to understand animal physiology. All the existing methods to study cell-specific gene expression require cellular or molecular sorting. This can be quite time-intensive, however, and has considerable detrimental effects on cells, leading to limited applications. Professor Eric Miska’s Group at the Wellcome Trust Gurdon Institute have developed a new method, SLAM-ITseq, which enables scientists to study gene expression in a cell-specific manner through RNA labelling combined with a novel high-throughput sequencing method. Since SLAM-ITseq eliminates the need for cellular or molecular sorting, this method can be employed to study wider cell types in an animal in an easier, yet more accurate manner than ever before.
28 June 2018
One cause of cancer can be exposure to chemical carcinogens. Often we think of tobacco smoke, but widely-used preservatives in bacon can be broken down by the liver into a carcinogen called nitrosamine, which may contribute to cancers in the digestive track. In their new paper, Dr Duncan Odom's Group at the CRUK Cambridge Institute used a cohort of mice to characterise what kinds of mutations occur in liver cells after exposure to a closely related compound called diethyl nitrosamine (DEN). DEN has long been used to create liver tumours, and their study applied very recently developed high throughput sequencing technologies to look at how protein coding genes can be altered in both early and later stages of tumour development. Together, the authors revealed the precise locations on a handful of cancer genes that drive tumour development, as well as the overall molecular pathways affected in a widely used model for chemically induced liver cancer.
13 June 2018
Aurora kinase A is regarded as a promising therapeutic target in cancers, but there is still much to discover about its roles in the cell, and how these contribute to cancer progression. A new study from Dr Rhys Grant et al. in Dr Catherine Lindon's Group at the Department of Pharmacology, University of Cambridge, shows that one of these roles concerns the organization of the mitochondrial network, the energy factory of the cell, which in the presence of excess Aurora A fragments into smaller units that are thought to function less efficiently. Fragmented mitochondria are a hallmark of cancerous cells, whose metabolism is less reliant on mitochondria than healthy cells. The finding that Aurora A is targeted to mitochondria raises exciting new questions about this key player that extend beyond its well-described role in controlling cell division to a more generalized role in cytoplasmic organization and metabolism.