Dr Alessandro Esposito

University of Cambridge

University departments
MRC Cancer Unit
University institutes
Hutchison MRC Research Centre

Position: Senior Investigator Scientist
Personal home page: http://www.quantitative-microscopy.org
Email:   Public email address:  ae275@mrc-cu.cam.ac.uk

PubMed journal articles - click here

Dr Alessandro Esposito is pleased to consider applications from prospective PhD students.

Research description

Our long-term goal is to understand how networks of biochemical reactions cooperate to the maintenance of cellular functional states and cellular homeostasis. More specifically, we are studying how biochemical networks encode for cellular decisions underlying cell and tissue homeostasis and how oncogenes contribute to early tumour initiation and promotion by reprogramming these processes. We study how cells attempt to repair DNA damage, how mutations arising from DNA lesions induce loss of homeostatic control and how we can improve our early detection of cancer, focusing on the following research areas:
- the study of checkpoint signalling and the DNA damage response (DDR) with particular interest on their heterogeneous response among a clonal population of cells;
- the study of how oncogenic signalling triggers cancer-associated phenotypes by reprogramming signalling and metabolic networks while avoiding cell-autonomous and non-cell-autonomous tumour suppressive mechanism;
- the translation of the technologies and knowledge developed for these studies to early detection and intervention;
- a transdisciplinary programme of research aimed to establish a ‘live single-cell systems biology of cellular function and cell decision’.

More information can be found in our lab webpage: https://quantitative-biology.org
And the CRUK funded OncoLive project: https://oncolive.online
Or on my twitter feed: https://twitter.com/alesposito75

Research Programme
Early Detection
Secondary Programme
Cell and Molecular Biology
Methods and technologies
Biosensor
Cell culture
Confocal microscopy
Fluorescence microscopy
Imaging
Microscopy
Statistical analysis
Tumour type interests
Colorectal
Lung
Pancreas
Keywords
non-genetic heterogeneity
carcinogenesis
oncogenic signalling
quantitative biology
KRAS
DNA damage
biochemical imaging
ae275
Recent publications:
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Key publications

De S, Campbell CJ, Venkitaraman AR*, Esposito A*, “Pulsatile MAPK signalling modulates p53 activity to control cell fate decisions at the G2 checkpoint for DNA damage”, Cell Reports (in press)

Esposito A and Venkitaraman AR (2019), “Enhancing biochemical resolution by hyper-dimensional imaging microscopy”, Biophys. J. 116(10):1815-1822

Haas KT, Lee M, Esposito A* and Venkitaraman AR*, “Single-molecule localization microscopy reveals molecular transactions during RAD51 filament assembly at cellular DNA damage sites”, Nucleic Acids Research 46(5):2398–2416

Liang H, Esposito A, De S, Ber S, Collin P, Surana U, Venkitaraman AR, Homeostatic control of the G2 checkpoint via polo-like kinase 1 engenders non-genetic heterogeneity in its fidelity and timing, Nat Comms, 5, 4048

Esposito A, Choimet JB, Skepper JN, Mauritz JMA, Lew VL, Kaminski CF, Tiffert T, “Quantitative imaging of human red blood cells infected with Plasmodium falciparum“, Biophys. J., 99(3):953-960

Esposito A, Dohm CP, Bähr M and Wouters FS, “Unsupervised Fluorescence Lifetime Imaging Microscopy for High-Content and High-Throughput Screening”. Mol. Cell. Proteomics 6:1446-1454

Esposito A, Gerritsen HC, Oggier T, Lustenberger F, Wouters FS, “Innovating lifetime microscopy: a compact and simple tool for the life sciences, screening and diagnostics”. J. Biomed. Opt. 11(3):34016-34024