Dr Guozhen Wang

MRC Laboratory of Molecular Biology

NHS or other affiliations: 
Medical Research Council Laboratory of Molecular Biology

Position: Career Development Fellow
Personal home page: http://www2.mrc-lmb.cam.ac.uk/group-leaders/a-to-g/alan-fersht

PubMed journal articles - click here

Research description: 

We work on the aggregation mechanism of the tumor suppressor protein, p53, and developing novel anti-cancer drugs that function by inhibiting aggregation of mutant p53.

Research Programme or Virtual Institute: 
Fundamental Biology of Cancer
Methods and technologies: 
Cell culture
Confocal microscopy
Fluorescence microscopy
HPLC/FPLC
Immunohistochemistry
Microscopy
PCR
Protein biochemistry
Protein purification
Recombinant protein expression
RNAi
Statistical analysis
Other
Tumour type interests: 
Other
Keywords: 
Biophysical methods (Fluorimetry
UV
DSF
DSC
ITC
Analytical Ultracentrifugation
Stoppped-flow
Anisotropy)
Electron Microscopy
Site-directed mutagenesis
gw387
Recent publications:
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Key publications: 

Peer-Reviewed Journal Articles: GuoZhen Wang and Alan Fersht. Mechanism of initiation of aggregation of p53 revealed by ?-value analysis. Proc. Natl. Acad. Sci. USA 112(8): 2437-2442 (2015) GuoZhen Wang and Alan Fersht. Propagation of aggregated p53: Cross-reaction and coaggregation vs. seeding. Proc. Natl. Acad. Sci. USA 112(8): 2443?2448 (2015) GuoZhen Wang and Alan Fersht. First-order rate-determining aggregation mechanism of p53 and its implications. Proc. Natl. Acad. Sci. USA 109 (34): 13590?13595 (2012) Rainer Wilcken, GuoZhen Wang, Frank M Boeckler, and Alan R Fersht. Kinetic mechanism of p53 oncogenic mutant aggregation and its inhibition. Proc. Natl. Acad. Sci. USA 109 (34): 13584?13589 (2012) (co-first author) GuangJie Han, XiaoYan Dong, Lin Zhang, LiTang Fu, GuoZhen Wang, Yan Sun. Facilitated oxidative refolding of ribonuclease A from inclusion bodies with a new redox system. Biochemical Engineering Journal 69, 106-112 (2012) Guo-Zhen Wang, Xiao-Yan Dong, Yan Sun. Ion-exchange resins greatly facilitate refolding of likely charged proteins at high concentrations. Biotechnology and Bioengineering 108(5): 1068-1077 (2011) Guo-Zhen Wang, Xiao-Yan Dong, Yan Sun. Acyl cystamine: small molecular foldase mimics accelerating oxidative refolding of disulfide-containing proteins. Biotechnology Progress 27(2): 377-385 (2011) Guo-Zhen Wang, Xiao-Yan Dong, Yan Sun. Peptide disulfides CGC and RKCGC facilitate oxidative protein refolding. Biochemical Engineering Journal 55(3): 169-175 (2011) Guo-Zhen Wang, Xiao-Yan Dong, Yan Sun. The role of disulfide bond formation in the conformational folding kinetics of denatured/reduced lysozyme. Biochemical Engineering Journal 46:7-11 (2009) PATENTs           Yan Sun, Guo-Zhen Wang, Qing-Hong Shi, Xiao-Yan Dong. Method of protein refolding with ion exchange resins and the application of the same. US patent NO. US 8624005 B2 (Granted) Yan Sun, Guo-Zhen Wang, Qing-Hong Shi, Xiao-Yan Dong. Small molecular mimics of oxidoreductase, N-alkyl cystamine: preparation and application. CN patent NO. 201010276124.4 (Granted) Yan Sun, Guo-Zhen Wang, Qing-Hong Shi, Xiao-Yan Dong. Ion exchange resins facilitate protein renaturation. CN patent NO. 201010507365.5 (Granted) Yan Sun, Guo-Zhen Wang, Qing-Hong Shi, Xiao-Yan Dong. Small peptides with intramolecular disulfide bond facilitate protein oxidative folding. CN patent NO. 201110037780.3 (Granted)