Professor Paul J Lehner
Personal home page: http://www.cimr.cam.ac.uk/investigators/lehner/index.html
PubMed journal articles - click here
Professor Paul J Lehner is pleased to consider applications from prospective PhD students.
We study MHC I molecules and other critical cell surface receptors. We showed that the cancer-causing herpesviruses (KSHV) pirated ubiquitin E3 ligases from their vertebrate hosts. These ligases ubiquitinate and downregulate critical cell surface receptors and teach us about receptor regulation, required for all growth factor receptors. Indeed E3 ligases have emerged as an important means of regulating receptor cell surface expression ? critical in many forms of human cancer. Our laboratory studies: (i)mechanisms used by viruses to evade immune recognition, particularly MHC class I molecules. (ii)the role of ubiquitin in the regulation of cell surface receptors, including the use of siRNA screens to identify the key enzymes of the ubiquitination pathway regulating these receptors. (iii)the use of quantitative proteomics (SILAC) to probe cell surface receptor regulation. (iv)components of the endosomal sorting machinery used by internalised, ubiquitinated cell surface receptors.
SILAC quantitative proteomics
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Thomas, M, Boname, JM, Field, S, Nejentsev, S, Salio, M, Cerundolo, V, Wills M, Lehner PJ. (2008). Downregulation of NKG2D and NKp80 ligands by Kaposi?s sarcoma-associated herpesvirus K5 protects against NK cell cytotoxicity Proc Natl Acad Sci 105:1656-61 Floto, RA, MacAry, PA, Boname, JM, Mien, TS, Kampmann, B, Hair, JR, Huey, OS, Houben, EN, Pieters, J, Day, C, Oehlmann, W, Singh, Smith, KGC and Lehner, PJ (2006) Dendritic Cell Stimulation by Mycobacterial HSP70 is Mediated Through CCR5. Science 314:454-8 Duncan, LM, Piper, S, Dodd, RB, Saville, MK, Sanderson, CM, Luzio JP and Lehner PJ (2006) Lysine-63 Linked Ubiquitination Is Required For Endolysosomal Degradation of Class I Molecules EMBO Journal 25:1635-1645