Children with cancer treated at Addenbrooke’s shown to benefit from genetic sequencing

Cambridge study shows that Whole Genome Sequencing improves diagnosis and treatment options for children with cancer.

A pilot study of 36 children treated at Cambridge University Hospitals NHS Foundation Trust has shown that whole genome sequencing can give a more accurate diagnosis or reveal new treatment options.

The study, part of the 100,000 Genomes Project, involved 36 children treated at Addenbrooke’s hospital in Cambridge and included 23 different types of tumours.

For each child, a sample of their tumour and blood were sent to a lab where the full DNA sequence could be read. Scientists then looked for genetic differences, called variants, that might offer clues about the specific cancer and which treatments might be most effective.

The study was carried out by members of our Paediatric Cancer Programme including Dr Amos Burke, Dr Matt Murray, Dr Sam Behjati and the Paediatric Oncology MDT Team at Addenbrooke's.

The results were presented at the National Cancer Research Institute (NCRI) Festival by Dr Patrick Tarpey, lead scientist for solid cancer in the East Genomic Laboratory Hub, based at Cambridge University Hospitals NHS Foundation Trust.

He said: “Whole genome sequencing can sometimes unravel unexpected results that may not have been considered via routine investigations. We’ve already learned a lot about using this type of test in our pilot study, both in terms of its benefits for children and in terms of how to get the best results.”

This process revealed several potentially important variants:

  • In two cases, the information refined children’s diagnosis
  • In four cases, it changed their diagnosis
  • In eight cases, it revealed new information about children’s prognoses (the likely course of their disease)
  • In two cases, it showed possible hereditary causes of the cancers
  • In seven cases, it revealed treatments that might not have been considered but were likely to be effective for treating the children.

In one of the seven cases, scientists found that two genes had become stuck together (known as gene fusion), and this was probably encouraging the tumour to grow. Knowing this meant that doctors could offer a different treatment, called a MEK inhibitor, that would not normally be used to treat this type of cancer.

Dr Tarpey said: “Our results from this relatively small pilot group of children with cancer, show how diagnosis and treatment can be improved. It suggests that offering whole genome sequencing to all children with cancer will provide more accurate diagnosis and prognosis, show whether there could be any hereditary cancer risk and help improve treatment options.”

The study also showed that some of the important variants were difficult to spot among all the data produced by whole genome sequencing. Their significance might have been missed without the careful analysis and interpretation provided by an expert panel called a Genomic Tumour Advisory Board.

Dr Tarpey added: “As we scale up whole genome sequencing to complement current standard-of-care testing for children with cancer, we need to ensure we optimise all the steps in this process to reduce turnaround times and keep costs down.”

Dr Amos Burke, Consultant Paediatric Oncologist at CUH commented: ““Being able to offer whole genome sequencing to all families of a child with cancer is a major step forward in allowing us to make the best diagnosis and to deliver the best treatment.

“Already we are seeing these results pointing to additional new therapies for some children that we would not have known about before. We will continue to discover more that can help to cure childhood cancer and as this new approach rolls out across the country, we look forward to seeing this as ‘standard-of-care’ for all children with cancer wherever they live.”

Dr Julia Chisholm is Chair of the NCRI’s Children’s Group, based at The Royal Marsden NHS Foundation Trust and The Institute of Cancer Research, London, UK, and was not involved in this research. She said: “Cancer is rare in children and thanks to research and better treatments, survival rates in the UK have doubled since the 1970s.

“This research shows that it’s feasible and beneficial to analyse the whole genetic code in children diagnosed with cancer. Whole genome sequencing helps us to be more precise in tumour diagnosis and to tailor treatments to suit individual patients as accurately as possible. As this innovation is introduced more widely, we hope that even more children will survive cancer.”

Patrick’s presentation is available to watch all week at the #NCRIFestival as part of the treatment short talks

8 Nov 2021